| Project title |
The development and stability of atheromatous plaque in a novel shear flow cell culture model |
| Summary |
This project investigates the effects of shear stress, branching, oxLDL and cytokine stimulation on gene expression in an in vitro cell culture model using microfluidic biochips (Celix). |
| Funding Agency |
Royal City of Dublin Hospital Trust |
| Programme |
Project Grants |
| Type of Project |
|
| Date from |
2010 |
| Date to |
2013 |
| Person Months |
72 |
|
|
| Project title |
The role of IL2 in the IL23/IL17 axis of inflammation in atherosclerosis |
| Summary |
This project extends previous work on the IL17 pathway and its interaction with other mediators of inflammation in the events leading to cardiovascular disease. High-throughput technologies are used to identify modifications in patterns of gene expression, ultimately with the aim of identifying the genetic basis of this variability. This is of interest because it has been shown that down-regulation of inflammation is a key aspect of the beneficial effects of statin therapy. |
| Funding Agency |
Royal City of Dublin Hospital Trust |
| Programme |
Project Grants |
| Type of Project |
Co-PI, with Dr Ross Murphy (PI) |
| Date from |
2008 |
| Date to |
2010 |
| Person Months |
24 |
|
|
| Project title |
The IL23/IL17 axis of inflammation in genetic susceptibility to atherosclerosis |
| Summary |
The role of inflammation in atherosclerosis is well recognised, and atherosclerotic plaques contain a layer of macrophages, which are derived from circulating monocytes. T-lymphocytes have been detected in unstable atherosclerotic plaques, suggesting that T-cell activation is important in the inititation and destabilization of these plaques. Recent studies have identified the IL23/IL17 pathway, which is involved in chronic inflammatory conditions. This project aims to investigate the role of the IL23/IL17 pathway in acute coronary syndrome, an atherosclerotic disease.
|
| Funding Agency |
Royal City of Dublin Hospital Trust |
| Programme |
Project Grants |
| Type of Project |
Co-PI, with Dr Ross Murphy (PI) |
| Date from |
2007 |
| Date to |
2008 |
| Person Months |
12 |
|
|
| Project title |
Directed analysis of genomic risk regions in coeliac disease |
| Summary |
A number of genomic regions have been identified by linkage studies as harboring genetic risk factors for coeliac disease (gluten intolerance), a common auto-immune disorder. Numerous candidate genes are present within these genomic regions. This project aims to perform candidate gene analysis, concentrated in established linkage peaks, particularly on chromosomes 5 and 11. A haplotype tagging approach is used in order to efficiently assay the common haplotypic variation at these loci. |
| Funding Agency |
Health Research Board |
| Programme |
Basic Research Grant |
| Type of Project |
Collaboration, with Dr Ross McManus (PI) |
| Date from |
2006 |
| Date to |
2008 |
| Person Months |
36 |
|
|
| Project title |
Fine scale mapping and identification of a major coeliac disease susceptibility gene on chromosome 5q |
| Summary |
The chromosomal region 5q31 has consistently been linked to coeliac disease (gluten intolerance) susceptibility in multiple populations. This finding has been replicated in numerous populations. However, despite intense research effort, the causative variant has not been identified. The aim of the project is to fine map the region in a large Irish case-control sample in order to identify the gene responsible for the chromosome 5q linkage peak in coeliac disease. More recent developments in the field have expanded this project to include analysis of other genomic regions, in addition to chromosome 5q. |
| Funding Agency |
Science Foundation Ireland |
| Programme |
Research Frontiers |
| Type of Project |
Collaboration, with Dr Ross McManus (PI) |
| Date from |
2006 |
| Date to |
2009 |
| Person Months |
36 |
|
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Hüffmeier U, Uebe S, Ekici AB, Bowes J, Giardina E, Korendowych E, Juneblad K, Apel M, McManus R, Ho P, Bruce IN, Ryan AW, Behrens F, Lascorz J, Böhm B, Traupe H, Lohmann J, Gieger C, Wichmann HE, Herold C, Steffens M, Klareskog L, Wienker TF, Fitzgerald O, Alenius GM, McHugh NJ, Novelli G, Burkhardt H, Barton A, Reis A, "Common variants at TRAF3IP2 are associated with susceptibility to psoriatic arthritis and psoriasis", Nature Genetics, 42, (11), 2010, p996 - 1000
Notes: [PMID: 20953186 ] |
Genetic Analysis of Psoriasis Consortium & the Wellcome Trust Case Control Consortium 2, Strange A, Capon F, Spencer CC, Knight J, Weale ME, Allen MH, Barton A, Band G, Bellenguez C, Bergboer JG, Blackwell JM, Bramon E, Bumpstead SJ, Casas JP, Cork MJ, Corvin A, Deloukas P, Dilthey A, Duncanson A, Edkins S, Estivill X, Fitzgerald O, Freeman C, Giardina E, Gray E, Hofer A, Hüffmeier U, Hunt SE, Irvine AD, Jankowski J, Kirby B, Langford C, Lascorz J, Leman J, Leslie S, Mallbris L, Markus HS, Mathew CG, McLean WH, McManus R, Mössner R, Moutsianas L, Naluai AT, Nestle FO, Novelli G, Onoufriadis A, Palmer CN, Perricone C, Pirinen M, Plomin R, Potter SC, Pujol RM, Rautanen A, Riveira-Munoz E, Ryan AW, Salmhofer W, Samuelsson L, Sawcer SJ, Schalkwijk J, Smith CH, Ståhle M, Su Z, Tazi-Ahnini R, Traupe H, Viswanathan AC, Warren RB, Weger W, Wolk K, Wood N, Worthington J, Young HS, Zeeuwen PL, Hayday A, Burden AD, Griffiths CE, Kere J, Reis A, McVean G, Evans DM, Brown MA, Barker JN, Peltonen L, Donnelly P, Trembath RC, "A genome-wide association study identifies new psoriasis susceptibility loci and an interaction between HLA-C and ERAP1", Nature Genetics, 42, (11), 2010, p985 - 990
Notes: [PMID: 20953190] |
Patel, K.D., Duggan, S.D., Currid, C.A., Gallagher, W.M., McManus, R., Kelleher, D., Murphy, R.T., Ryan, A.W., "High sensitivity cytokine detection in acute coronary syndrome reveals up-regulation of Interferon Gamma and Interleukin-10 post Myocardial Infarction", Clinical Immunology, 133, (2), 2009, p251 - 256
Notes: [PMID: 19665935] |
Patel, K.D., Murphy, R.T., White, M., Gasparro, D., Kelleher, D.P., Ryan, T., McManus, R., Ryan, A.W., "Interleukin 17: An unlikely marker of Acute Coronary Syndrome?", Atherosclerosis, 205, (1), 2009, p33 - 34
Notes: [PMID: 19131064] |
Ryan, A.W., Hughes, D., Tang, K., Kelleher, D., Ryan, T. McManus, R. and Stoneking, M. , "Natural selection and the molecular basis of electrophoretic variation at the coagulation F13B locus", European Journal of Human Genetics, 17, 2009, p219 - 227
Notes: [PMID: 18716611] |
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