Skip to main content

Trinity College Dublin, The University of Dublin

Menu Search


Trinity College Dublin By using this website you consent to the use of cookies in accordance with the Trinity cookie policy. For more information on cookies see our cookie policy.

      
Profile Photo

Dr. Ursula Bond

Associate Prof/Head of Department (Microbiology)


Dr. Ursula Bond, a Fellow of Trinity College Dublin, graduated from TCD with a degree in Biochemistry and was awarded a PhD in Molecular Biology by Washington University, St Louis. She conducted post-doctoral research at Yale University as an Anna Fuller Fellow before returning to Trinity College Dublin, taking up an academic role in the Department of Microbiology. Dr. Bond is a world leader in the field of industrial yeast genetics and carries out research into the genetic make-up of yeasts used in the fermentation of beers, wines and whiskeys. As an expert in the field, Dr.. Bond is highly regarded as a consultant for several major breweries. Dr.Bond is developing bespoke yeast strains that produce novel antimicrobial peptides to prevent beer spoilage by bacteria and has developed new strains of yeasts for the production of bioethanol from waste biomass. She also researches fundamental molecular biological questions using the model microorganism, the Baker's yeast, uncovering important discoveries in the molecular mechanism of RNA biogenesis in yeasts.
  Cancer Biology   Cell cycle regulation in yeasts   Gene Expression in yeasts   Genetic Engineering   Genetics   Genome structures in brewery yeasts   Molecular Biology   Molecular Genetics   Molecular markers and recognition   mRNA metabolism   Nuclei acids, polynucleotides, protein synthesis   Tumour markers
 Natural peptides to enhance food quality and safety
 Preventing Beer Spoilage in Lager Fermentations: Optimisation of the production of the antimicrobial defensin peptides in lager strains of yeast, a natural defense against beer-spoiling bacteria
 Bioengineering bespoke yeast strains to convert biomass to biofuel.
 Antimicrobial peptide preventing beer spoilage wit neutraceutic potential
 Synthetic Immuno-modulatory Ppetides for Cancer Therapy

Page 1 of 2
Details Date
External Examiner BSc degree in Biotechnology, University College cork 2017-2020
Member of International Peer Review Panel of Danish Research Council: Technology and Production Sciences in the field of biotechnology and biotechnological production 2015-2017
Steering Committee on FIRM awards, Department of Agriculture, Food and the Marine 2010-2012
Details Date From Date To
Member of the American Association for the Advancement of Science
Member of the American Society of Microbiologists
Member of the RNA Society
Member Society for General Microbiology
Chandre Monerawela and Ursula Bond, Brewing up a storm: The genomes of lager yeasts and how they evolved, Biotechnology Advances, 2017, p1-8 , Notes: [pii: S0734-9750(17)30021-6.], Journal Article, PUBLISHED  DOI  URL
Monerawela, C and U. Bond, Recombination Sites on Hybrid Chromosomes in S. pastorianus share Common Sequence Motifs and define a Complex Evolutionary Relationship between Group I and II Lager Yeasts, FEMS Yeast Research, 17, (5), 2017, p1 - 12, Notes: [ https://doi.org/10.1093/femsyr/fox047], Journal Article, PUBLISHED  URL
Monerawela, C and U. Bond, The hybrid genomes of Saccharomyces pastorianus-a current perspective, YEAST, 2017, Review Article, IN_PRESS
Chandre Monerawela , Brewing up a Mystery: The origins and recombination events in lager yeasts, Trinity College Dublin, 2017, Thesis, PUBLISHED
Kraszewska J, Beckett M.C, James T.C, Bond U, Comparative analysis of the antimicrobial activities of plant defensin-like and ultrashort peptides against food-spoiling bacteria, Applied and Environmental Microbiology, 82, (14), 2016, p4288 - 4298, Notes: [Cited By :1 Export Date: 13 December 2016], Journal Article, PUBLISHED  DOI  URL
Thery T, Tharappel J.C, Kraszewska J, Beckett M, Bond U, Arendt E.K, Antifungal activity of a synthetic human β-defensin 3 and potential applications in cereal-based products, Innovative Food Science and Emerging Technologies, 38, 2016, p160 - 168, Notes: [Export Date: 13 December 2016], Journal Article, PUBLISHED  DOI  URL
Monerawela, C., James, T.C., Wolfe, K., and U. Bond, Loss Of Lager Specific Genes And Subtelomeric Regions Define Two Different Saccharomyces cerevisiae Lineages for Saccharomyces pastorianus Group I and II Strains., FEMS Yeast Research, 15, 2015, p1 - 11, Journal Article, PUBLISHED  TARA - Full Text  DOI
Kricka, W., Fitzpatrick, J., and U. Bond, Challenges for the Production of Bioethanol from Biomass using Recombinant Yeasts, Advances in Applied Microbiology, 92, 2015, p89 - 125, Notes: [Review Article], Journal Article, PUBLISHED  DOI
Kricka, W., Fitzpatrick, J., T.C. James and U. Bond, Engineering Saccharomyces pastorianus for the co-utilisation of xylose and cellulose from biomass., Microbial Cell Factories, 14, (61), 2015, p1-11 , Notes: [ doi: 10.1186/s12934-015-0242-4.], Journal Article, PUBLISHED  TARA - Full Text  DOI
Lall, P., Lindsay, A.J., Hanscom, S., (...), McCaffrey, M.W., Khan, A.R., Structure-function analyses of the interactions between Rab11 and Rab14 small GTPases with their shared effector Rab coupling protein (RCP), Journal of Biological Chemistry, 290, (30), 2015, 18817-18832 , Notes: [ ], Journal Article, PUBLISHED  TARA - Full Text  DOI
  

Page 1 of 8
Bond, U., Monerawela, C., and I. Sugre. (2015) , Impact of unique lager-specific genes on phenotypes in Saccharomyces pastorianus. , YEAST, International Congress on Yeast genetics and Molecular Biology, Levico Therme, Italy, 32, 2015, Conference Paper, PUBLISHED
U. Bond, J. Usher and T.C. James , Brewing yeasts possess dynamic genomes that undergo rearrangements and chromosome loss in response to stress., YEAST, International Congress on Yeast genetics and Molecular Biology, Serrento Italy, 2007, 24, 2007, Conference Paper, PUBLISHED
Donnelly, D., P. Brosnan, and U. Bond. , The active response to stress., A New Era of Opportunity, European Brewery Monograph , Proceedings of the European Brewery Convention Symposium, Nutfield, U.K., , 1999, 228, 1999, Conference Paper, PUBLISHED
Donnelly, D.; Brosnan, P., U. Bond , Stress responses in brewing yeasts , Journal of the Institute of Brewing , 2nd Technical Meeting of the European Brewery Convention Brewing Science Group , Edinburgh, Scotland, 1998, 105, (1), 1998, pp8-, Conference Paper, PUBLISHED
Agell, N., Bond. U and S.J Schlesinger , Proteolytic processing of polyubiquitin, polyubiquitin J. Cell Biology , 105, 1987, Conference Paper, PUBLISHED
Thomas, G.H., Siegfried, E., Bond. U, and S. Elgin. , Hypersensitivity sites in Hsp26 Promoter, Genetics, 113, (1), 1986, Conference Paper, PUBLISHED
Siegfried, E., Bond, U., Elgin, SCR., Supercoil-dependent s1-nuclease sensitive sites in the drosophila locus-67B199 , Journal of Cell Biology., 4, 1986, Conference Paper, PUBLISHED
James T C, Maack C C, Bond U, et al , Vitellogenin coding sequences in insects are conserved , Journal of Cellular Biochemistry , 6, 1982, Conference Paper, PUBLISHED

  

Award Date
Gerti T Cori award in Biochemistry 1984
Anna Fuller Cancer Fellowship 1987
Fellow of Trinity College Dublin May 2007
Ursula Bond, Eukaryotic Gene Expression and Biotechnology Laboratory, Department of Microbiology. The Genomes of Lager Yeasts My research group focuses on understanding the complex genetic make-up of yeasts used in the production of lager beer. Saccharomyces pastorinaus is a hybrid species that emerged just 500-600 years ago as a result of a fusion between of two yeast species, Saccharomyces cerevisiae and Saccharomyces eubayanus. Given the importance of these yeasts in the global beer industry, we are addressing questions such as . What is the genetic composition of the genomes of lager yeasts? We discovered that the lager yeast can be divided into two broad groups (I and II) based on gene content and structure and that the parent genomes have recombined at specific chromosomal locations to create a unique set of hybrid chromosomes. We identified a common sequence motif at the recombination epicentres, indicative of a common molecular mechanism controlling these recombination events. In fact, we discovered that recombination at these sites is induced in response to environmental stress. Thus, stresses encountered during industrial fermentations play an important role in the evolution of these yeasts. . How did this species evolve? By analysing the genomes of hundreds of yeast species, we search for the ancestral origins of the species. We discovered that the genomes of lagers yeasts contain genetic information related to modern day Ale and Stout yeasts, leading us to hypothesis that the two lager yeast types arose by sequential rounds of hybridisation firstly between S. eubayanus and an Ale yeasts and subsequently with a Stout yeasts giving rise to Group I and II lager yeasts respectively. This seminal discovery was recently highlighted in several articles published in The Irish Times in 2014 and 2015. . Unique genetic characteristics of lager yeasts. Through mining the genomes of lager yeasts, we identified several genes unique to the species. These include hybrid genes emerging as a result of the recombination between parental chromosomes as well as genes located at the tips of chromosomes have been lost in most other yeast species but preferentially retained in lager yeasts. Secondly, as lager yeasts are polyploid, they contain multiple copies of the same gene as well as gene variants due to the presence of the two parent genomes. Through analyzing the effects of the gene products (and gene dosage) on the biochemistry and physiology of the cell, we aim to decipher the complex algorithm of gene expression leading to the unique characteristics of beer produced by lager yeasts. 2. Improving yeast strains. We are interested in generating industrial yeasts with improved characteristics such as increased ethanol tolerance, improved fermentation capacity and for producing new biological products using adaptive evolution, synthetic biology and metabolic engineering approaches. Specifically, we have developed (i) Strains of lager yeasts that can metabolise both xylose and cellulose, the major components of plant biomass, in a project aimed at using alternative environmentally sustainable energy sources for biofuel production. (ii) Lager yeasts that produce their own natural antibacterial peptides, by expressing genes encoding defensin-like peptides from plant sources. We examine how the co-expression of multiple peptides on artificial chromosomes can provide natural protection against beer-spoiling bacteria. 3. RNA Production during the Cell Cycle in Yeasts. We continue to study fundamental molecular biological questions in yeasts, specifically relating to the biogenesis RNA. We have contributed several seminal discoveries in this field, uncovering important molecular mechanism of histone and small nuclear RNA biogenesis. Specifically, we are interested in why such diverse mechanisms have emerged in yeasts and human cells to control a biogenesis of histone messenger RNAs.