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Dr. Ursula Bond

Associate Professor (Microbiology)


  Cancer Biology   Cell cycle regulation in yeasts   Gene Expression in yeasts   Genetic Engineering   Genetics   Genome structures in brewery yeasts   Molecular Biology   Molecular Genetics   Molecular markers and recognition   Nuclei acids, polynucleotides, protein synthesis   Tumour markers   mRNA metabolism
Preventing Beer Spoilage in Lager Fermentations: Optimisation of the production of the antimicrobial defensin peptides in lager strains of yeast, a natural defense against beer-spoiling bacteria
Improving yeast strains with increase ethanol production
Bioengineering bespoke yeast strains to convert biomass to biofuel.
Antimicrobial peptide preventing beer spoilage wit neutraceutic potential
Synthetic Immuno-modulatory Ppetides for Cancer Therapy

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Details Date From Date To
Member Society for General Microbiology
Member of the American Association for the Advancement of Science
Member of the American Society of Microbiologists
Member of the RNA Society
Fitzpatrick, J. Kricka, W., James, T.C. and U. Bond, Expression of three Trichoderma reesei cellulase genes in Saccharomyces pastorianus for the development of a two-step process of hydrolysis and fermentation of cellulose , Journal of Applied Microbiology, epub 03/14, 2014, Journal Article, PUBLISHED  DOI
Generation of New Genotypic and Phenotypic Features in Artificial and Natural Yeast Hybrids (2014) Pfliegler, W.P., Atanasova, L., Karanyicz, E., Sipiczki, M. Bond, U., Druzhinina,I.S., Sterflinger, K., and K. Lopandic, Generation of New Genotypic and Phenotypic Features in Artificial and Natural Yeast Hybrids, Food Technology and Biotechnology , 52, (1), 2014, p46 - 57, Journal Article, PUBLISHED  TARA - Full Text
Kricka, W., Fitzpatrick, J. and U. Bond. , Metabolic engineering of yeasts by heterologous enzyme production for degradation of cellulose and hemicellulose from biomass: a perspective, Frontiers in Microbiology, 5, (174), 2014, p1-11 , Review Article, PUBLISHED  DOI
James TC, Gallagher L, Titze J, Bourke P, Kavanagh J, Arendt E, Bond U., In situ production of human β defensin-3 in lager yeasts provides bactericidal activity against beer-spoiling bacteria under fermentation conditions., Journal of Applied Microbiology, 116, (2 ), 2013, p368 - 379 , Journal Article, PUBLISHED  DOI
Suzanne Beggs, Tharappel C. James and Ursula Bond, The PolyA tail length of yeast histone mRNAs varies during the cell cycle and is influenced by Sen1p and Rrp6p, Nucleic Acids Research, 40, (6), 2012, p2700 - 2711, Journal Article, PUBLISHED  TARA - Full Text
James, T.C. and U, Bond, Molecular Mimics of the Tumour Antigen MUC1, PLOS One, 7, (11), 2012, p1-10 , Journal Article, PUBLISHED  TARA - Full Text  URL
Siebke C, James TC, Cummins R, O'Grady T, Kay E, Bond U., Phage display biopanning identifies the translation initiation and elongation factors (IF1α-3 and eIF-3) as components of Hsp70-peptide complexes in breast tumour cells., Cell Stress Chaperones, 17, (2), 2012, p145-156 , Notes: [PubMed ID: 22002548], Journal Article, PUBLISHED  DOI
The Genomes of Lager Yeasts in, editor(s)Laskin, A., Sariaslani, S. and Gadd, G. , Advances in Applied Microbiology, Academic Press, 2010, pp159 - 182, [Bond, U.], Book Chapter, PUBLISHED
Querol, A and Bond U, The complex and dynamic genomes of industrial yeasts. , FEMS Microbiol Lett. , 293, (1), 2009, p1 - 10, Journal Article, PUBLISHED  DOI
Usher, J and Bond, U., Recombination between Homeologous Chromosomes in Lager Yeasts leads to Loss of Function of the Hybrid GPH1 Gene., Applied and Environmental Microbiology, 75, (13), 2009, p4573 - 4579, Journal Article, PUBLISHED  DOI
  

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Award Date
Anna Fuller Cancer Fellowship 1987
Gerti T Cori award in Biochemistry 1984
Ursula Bond, Eukaryotic Gene Expression and Biotechnology Laboratory, Department of Microbiology. 1. Characterisation of Lager Yeasts The yeast Saccharomyces cerevisiae is an ideal model system to advance our understanding of the rules and principles of cellular systems. We are currently conducting a systematic analysis of the genome and transcriptome of the industrially important strains of yeasts. We discovered that the genomes of lager yeasts are extremely dynamic and undergo rearrangements in response to environmental stress. Expanding on our knowledge of industrial genomes, we are interested in generating industrial yeasts with improved characteristics such as increased ethanol tolerance and improved fermentation capacity, using an adaptive evolution approach. We have also developed yeast strains expressing natural antimicrobial peptides which can prevent the growth of beer-spoiling bacterial in industrial fermentations. 2. Biomass to Biofuel. As the world enters a post fossil fuel era, there is an urgent need to identify alternative environmentally sustainable energy sources. One potential energy source is biomass such as grasses, woody plants, or industrial waste. Energy in biomass is stored as lignocellulose which is broken down by enzymes called cellulases. A current goal in the biofuel industry is to generate microorganisms that can breakdown the cellulose in biomass into a sugars which can be fermented into ethanol, a process called consolidated bioprocessing. Industrial yeasts such as those used in the brewing and distilling industries are robust producers of ethanol, however they do not possess the enzymes to degrade cellulose. Our laboratory have developed a series of yeast strains expressing the genes encoding for the cellulase enzymes from the fungus Trichoderma reesei. The yeast strains secrete the recombinant enzymes into the medium and are capable of degrading cellulose. We are currently optimising for the production of the cellulase enzymes and generating new strains capable of degrading both cellulose and hemicellulose. 3. RNA Production during the Cell Cycle in Yeasts We have been analysing the mechanisms controlling the synthesis of histone messenger RNAs during the yeast cell cycle. The ends of histone mRNAs in higher eukaryotes such as humans was very unusual and lack a characteristic 'PolyA' tail. This unusual structure is extremely important to the cell cycle regulation of histone mRNAs. Surprisingly yeast histone mRNAs do have a PolyA tail and yet the mRNAs are also cell cycle regulated. Therefore, an interesting evolutionary question arises as to why such diverse mechanisms have emerged in yeasts and human cells to control a very important regulatory mechanism. Our current research focusses on the role of proteins such as Sen1p and Rrp6 in the degradation of histone mRNAs during the cell cycle. 4. Stress and Cancer A key requirement for the development of cancer immunotherapy is the identification of tumour-associated antigens that are differentially or exclusively expressed on the tumour and recognized by the host immune system. Unfortunately, immune responses to such antigens are often muted or lacking due to the antigens being recognized as "self", by the tumour environment and regulation of immune cells within. To circumvent the lack of immune responses to tumour antigens, we have devised a strategy to develop potential synthetic immunogens using a strategy, termed mirror image phage display. Using this approach we have developed a series of molecular mimics of known and unknown tumour antigenss and show that the mimic molecules display characteristics such as immunstimulation of T-cells in vitro. These molecular mimics cross-stimulate T-cells previously stimulated with the native antigen. Thus mimic peptides are good candidates for the generation of a cancer vaccine.