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Dr. Ursula Bond

Associate Prof/Head of Department (Microbiology)


  Cancer Biology   Cell cycle regulation in yeasts   Gene Expression in yeasts   Genetic Engineering   Genetics   Genome structures in brewery yeasts   Molecular Biology   Molecular Genetics   Molecular markers and recognition   mRNA metabolism   Nuclei acids, polynucleotides, protein synthesis   Tumour markers
 Natural peptides to enhance food quality and safety
 Preventing Beer Spoilage in Lager Fermentations: Optimisation of the production of the antimicrobial defensin peptides in lager strains of yeast, a natural defense against beer-spoiling bacteria
 Improving yeast strains with increase ethanol production
 Bioengineering bespoke yeast strains to convert biomass to biofuel.
 Antimicrobial peptide preventing beer spoilage wit neutraceutic potential

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Details Date From Date To
Member of the American Association for the Advancement of Science
Member of the American Society of Microbiologists
Member of the RNA Society
Member Society for General Microbiology
Monerawela, C., James, T.C., Wolfe, K., and U. Bond, Loss Of Lager Specific Genes And Subtelomeric Regions Define Two Different Saccharomyces cerevisiae Lineages for Saccharomyces pastorianus Group I and II Strains., FEMS Yeast Research, 15, 2015, p1 - 11, Journal Article, PUBLISHED  DOI
Kricka, W., Fitzpatrick, J., T.C. James and U. Bond, Engineering Saccharomyces pastorianus for the co-utilisation of xylose and cellulose from biomass., Microbial Cell Factories, 14, (61), 2015, p1-11 , Notes: [ doi: 10.1186/s12934-015-0242-4.], Journal Article, PUBLISHED
Kricka, W., Fitzpatrick, J., and U. Bond, Challenges for the Production of Bioethanol from Biomass using Recombinant Yeasts, Advances in Applied Microbiology, 92, 2015, p89 - 125, Notes: [doi: 10.1016/bs.aambs.2015.02.003], Review Article, PUBLISHED
James TC, Gallagher L, Titze J, Bourke P, Kavanagh J, Arendt E, Bond U., In situ production of human β defensin-3 in lager yeasts provides bactericidal activity against beer-spoiling bacteria under fermentation conditions., Journal of Applied Microbiology, 116, (2 ), 2014, p368 - 379 , Journal Article, PUBLISHED  DOI
Fitzpatrick, J. Kricka, W., James, T.C. and U. Bond, Expression of three Trichoderma reesei cellulase genes in Saccharomyces pastorianus for the development of a two-step process of hydrolysis and fermentation of cellulose , Journal of Applied Microbiology, epub 03/14, 2014, p96-108 , Journal Article, PUBLISHED  DOI
Generation of New Genotypic and Phenotypic Features in Artificial and Natural Yeast Hybrids (2014) Pfliegler, W.P., Atanasova, L., Karanyicz, E., Sipiczki, M. Bond, U., Druzhinina,I.S., Sterflinger, K., and K. Lopandic, Generation of New Genotypic and Phenotypic Features in Artificial and Natural Yeast Hybrids, Food Technology and Biotechnology , 52, (1), 2014, p46 - 57, Journal Article, PUBLISHED  TARA - Full Text
Kricka, W., Fitzpatrick, J. and U. Bond. , Metabolic engineering of yeasts by heterologous enzyme production for degradation of cellulose and hemicellulose from biomass: a perspective, Frontiers in Microbiology, 5, 2014, p174-, Review Article, PUBLISHED  TARA - Full Text  DOI
Kricka, William, Biomass to biofuel: The engineering of Saccharomyces species for the co-fermentation of cellulose and xylose, Trinity College Dublin, 2014, Thesis, PUBLISHED
Ursula Bond, Tharappel C. James, Joanne Kavanagh, 'Preventing spoilage in alcohol fermentations ', US, US 13/482,223, 2013, Ursula Bond, James Tharappel C, Joanne Kavanagh, Patent, GRANTED  URL
Ursula Bond, Christina SIEBKE, Tharappel JAMES, 'BIOMARKERS FOR BREAST TUMOURS FROM Hsp70-ASSOCIATED PEPTIDES', World Intellectual Property Organisation, WO2013037714 A1, 2013, The Provost, Fellows, Foundation Scholars, And The Other Members Of Board, Of The College Of The Holy And Undivided Trinity Of Queen Elizabeth, Near Dublin, Patent, GRANTED  URL
  

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Award Date
Gerti T Cori award in Biochemistry 1984
Anna Fuller Cancer Fellowship 1987
Ursula Bond, Eukaryotic Gene Expression and Biotechnology Laboratory, Department of Microbiology. 1. Characterisation of Lager Yeasts The yeast Saccharomyces cerevisiae is an ideal model system to advance our understanding of the rules and principles of cellular systems. We are currently conducting a systematic analysis of the genome and transcriptome of the industrially important strains of yeasts. We discovered that the genomes of lager yeasts are extremely dynamic and undergo rearrangements in response to environmental stress. Expanding on our knowledge of industrial genomes, we are interested in generating industrial yeasts with improved characteristics such as increased ethanol tolerance and improved fermentation capacity, using an adaptive evolution approach. We have also developed yeast strains expressing natural antimicrobial peptides which can prevent the growth of beer-spoiling bacterial in industrial fermentations. 2. Biomass to Biofuel. As the world enters a post fossil fuel era, there is an urgent need to identify alternative environmentally sustainable energy sources. One potential energy source is biomass such as grasses, woody plants, or industrial waste. Energy in biomass is stored as lignocellulose which is broken down by enzymes called cellulases. A current goal in the biofuel industry is to generate microorganisms that can breakdown the cellulose in biomass into a sugars which can be fermented into ethanol, a process called consolidated bioprocessing. Industrial yeasts such as those used in the brewing and distilling industries are robust producers of ethanol, however they do not possess the enzymes to degrade cellulose. Our laboratory have developed a series of yeast strains expressing the genes encoding for the cellulase enzymes from the fungus Trichoderma reesei. The yeast strains secrete the recombinant enzymes into the medium and are capable of degrading cellulose. We are currently optimising for the production of the cellulase enzymes and generating new strains capable of degrading both cellulose and hemicellulose. 3. RNA Production during the Cell Cycle in Yeasts We have been analysing the mechanisms controlling the synthesis of histone messenger RNAs during the yeast cell cycle. The ends of histone mRNAs in higher eukaryotes such as humans was very unusual and lack a characteristic 'PolyA' tail. This unusual structure is extremely important to the cell cycle regulation of histone mRNAs. Surprisingly yeast histone mRNAs do have a PolyA tail and yet the mRNAs are also cell cycle regulated. Therefore, an interesting evolutionary question arises as to why such diverse mechanisms have emerged in yeasts and human cells to control a very important regulatory mechanism. Our current research focusses on the role of proteins such as Sen1p and Rrp6 in the degradation of histone mRNAs during the cell cycle. 4. Stress and Cancer A key requirement for the development of cancer immunotherapy is the identification of tumour-associated antigens that are differentially or exclusively expressed on the tumour and recognized by the host immune system. Unfortunately, immune responses to such antigens are often muted or lacking due to the antigens being recognized as "self", by the tumour environment and regulation of immune cells within. To circumvent the lack of immune responses to tumour antigens, we have devised a strategy to develop potential synthetic immunogens using a strategy, termed mirror image phage display. Using this approach we have developed a series of molecular mimics of known and unknown tumour antigenss and show that the mimic molecules display characteristics such as immunstimulation of T-cells in vitro. These molecular mimics cross-stimulate T-cells previously stimulated with the native antigen. Thus mimic peptides are good candidates for the generation of a cancer vaccine.