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Dr. Brenda McManus

Research Fellow (Dental Science)

 


  DNA   Drug Resistance   Epidemiology   Gene Cloning   Gene Expression   Genetic/Molecular epidemiology   Genomics   Molecular Cloning   Molecular population genetics   Nucleic Acids   Oral diseases and Oral medicine   Oral microbiology   Population structure and dynamics
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English Fluent Fluent Fluent
French Basic Basic Basic
McMANUS BA, COLEMAN DC, MOLECULAR EPIDEMIOLOGY. PHYLOGENY AND EVOLUTION OF Candida albicans, INFECTION GENETICS AND EVOLUTION, 21, (January 2014), 2014, p166 - 178, Notes: [19 November; Epub ahead of print], Journal Article, PUBLISHED  TARA - Full Text  DOI  URL
ABDULRAHIM MH, McMANUS BA, FLINT SR, COLEMAN DC, GENOTYPING Candida albicans FROM CANDIDA LEUKOPLAKIA AND NON-CANDIDA LEUKOPLAKIA SHOWS NO ENRICHMENT OF MULTILOCUS SEQUENCE TYPING CLADES BUT ENRICHMENT OF ABC GENOTYPE C IN CANDIDA LEUKOPLAKIA, PLOS ONE, 8, (9), 2013, p1-11:e73738 , Notes: [Published: September 18th 2013], Journal Article, PUBLISHED  TARA - Full Text  DOI  URL
Agwu E, Ihongbe JC, McManus BA, Moran GP, Coleman DC, Sullivan DJ, Distribution of yeast species associated with oral lesions in HIV-infected patients in Southwest Uganda., Medical Mycology, 50, 2012, p276 - 280, Journal Article, PUBLISHED
McMANUS BA, MAGUIRE, R., CASHIN, PJ, CLAFFEY N, FLINT, S, ABDULRAHIM MA AND COLEMAN, DC, ENRICHMENT OF MULTILOCUS SEQUENCE TYPING CLADE 1 WITH ORAL CANDIDA ALBICANS ISOLATES IN PATIENTS WITH UNTREATED PERIODONTITIS, JOURNAL OF CLINICAL MICROBIOLOGY , 50, (10), 2012, p3335-3344 , Notes: [8th August; Epub ahead of print], Journal Article, PUBLISHED  TARA - Full Text  DOI  URL
McMANUS, B.A., McGOVERN, E., MORAN, G.P., HEALY, C.M., NUNN, J., FLEMING, P., COSTIGAN, C., SULLIVAN, D.J., COLEMAN, D.C., MICROBIOLOGICAL SCREENING OF IRISH AUTOIMMUNE POLYENDOCRINOPATHY-CANDIDIASIS-ECTODERMAL DYSTROPHY (APECED) PATIENTS REVEALS PERSISTENCE OF CANDIDA ALBICANS STRAINS, GRADUAL REDUCTION IN SUSCEPTIBILITY TO AZOLES AND INCIDENCES OF CLINICAL SIGNS OF ORAL CANDIDIASIS WITHOUT CULTURE EVIDENCE, JOURNAL OF CLINICAL MICROBIOLOGY, 49, (5), 2011, p1879 - 1889, Journal Article, PUBLISHED  TARA - Full Text
Molecular Epidemiology of Candida species. in, editor(s)Ashbee, HR. & Bignell, EM. , Handbook of Pathogenic Yeasts., Berlin Heidelberg, Springer Verlag, 2010, pp19 - 39, [Moran, G.P., McManus, B.A., Coleman, D.C. & D.J. Sullivan], Notes: [Chapter 2 e-ISBN 978-3-642-03150-2], Book Chapter, PUBLISHED
COLEMAN DC, MORAN GP, McMANUS BA, SULLIVAN DJ, MECHANISMS OF ANTIFUNGAL DRUG RESISTANCE IN CANDIDA DUBLINIENSIS, FUTURE MICROBIOLOGY, 5, (6), 2010, p935-949 , Review, PUBLISHED  DOI  URL
McMANUS BA, SULLIVAN DJ, MORAN GP, D'ENFERT C, BOUGNOUX M-E, NUNN MA, AND COLEMAN DC, AVIAN-ASSOCIATED AND HUMAN ISOLATES OF Candida dubliniensis ARE GENETICALLY DISTINCT, EMERGING INFECTIOUS DISEASES, 15, (9), 2009, p1467-70 , Journal Article, PUBLISHED  TARA - Full Text  DOI  URL
McManus BA, Moran GP, Higgins JA, Sullivan DJ, Coleman DC, A Ser29Leu substitution in the cytosine deaminase Fca1p is responsible for clade-specific flucytosine resistance in Candida dubliniensis., Antimicrobial agents and chemotherapy, 53, (11), 2009, p4678-85 , Journal Article, PUBLISHED
McManus BA, Coleman DC, Moran G, Pinjon E, Diogo D, Bougnoux ME, Boreck√°-Melkusova S, Bujd√°kova H, Murphy P, d'Enfert C, Sullivan DJ., Multilocus sequence typing reveals that the population structure of Candida dubliniensis is significantly less divergent than that of Candida albicans., Journal of Clinical Microbiology, 46, (2), 2008, p652 - 664, Notes: [ ], Journal Article, PUBLISHED  TARA - Full Text
  


  

Collaborative investigation of Candida populations in Irish patients with Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Dystrophy (APECED). My most recent research focused Irish patients with APECED, an autosomal recessive genetic disease that often manifests itself in the form of chronic mucocutaneous candidiasis. The study was carried out over 5 years in collaboration with dental clinicians in the DDUH, aiming to characterise the Candida population present in this group, and to correlate clinical signs of candidiasis with microbiological evidence of such. The genetic relatedness of Candida albicans isolates recovered during sequential clinical assessments of these patients was investigated, as was the effect of prophylactic intermittent antifungal therapy on the susceptibility of these isolates to azole antifungals. This study highlighted the need for microbiological diagnosis of candidiasis prior to the commencement of antifungal therapy in order to prevent unnecessary azole therapy and the development of azole resistance. Previous research focused on the use of multilocus sequence typing (MLST) to characterise the population structure of the pathogenic yeast Candida dubliniensis, and to compare the evolutionary biology of C. dubliniensis with that of its closest relative, C. albicans. The application of similar techniques to C. dubliniensis isolates recovered from seagulls showed that such isolates were genetically distinct to those recovered from human sources. One of the three specific clades in the population structure of C. dubliniensis is characterised by high-level resistance to the antifungal drug 5-fluorocytosine. My research demonstrated that the primary cause of this clade-specific resistance is a recessive Ser29Leu substitution in the cytosine deaminase. Further research into the evolutionary biology of C. dubliniensis will involve the comparative analysis of single nucleotide polymorphisms (SNPs) throughout the complete genomes of ten C. dubliniensis isolates representative of each MLST clade. Comparison of such SNPs with those in C. albicans may identify reasons why, despite their close genetic relationship, C. dubliniensis is significantly less pathogenic than C. albicans.